生物活性 |
(Rac)-Indoximod (1-Methyl-DL-tryptophan) is an indoleamine 2,3-dioxygenase (IDO) inhibitor. Co-treatment with IFN-γ and (Rac)-Indoximod markedly reduces the activity of human cardiac myofibroblasts (hCMs) expressing α-SMA and induces apoptosis through up-regulating the IRF-1, Fas, and FasL genes[1].
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IC50 & Target[1] |
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体外研究
(In Vitro) |
(Rac)-Indoximod (1-Methyl-DL-tryptophan; 0.5 mM; 3 days) inhibits tryptophan depletion, thereby partially reversing the growth-inhibitory activity of IFN-γ, but eventually induces cell death regardless of tryptophan depletion[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line: |
Human cardiac myofibroblasts (hCMs) |
Concentration: |
0.5 mM |
Incubation Time: |
3 days |
Result: |
Growth retardation by IFN-γ was partially reversed on day 2, but cell viability was further reduced on day 3. |
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分子量 |
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Formula |
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CAS 号 |
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运输条件 |
Room temperature in continental US; may vary elsewhere.
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储存方式 |
4°C, protect from light
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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溶解性数据 |
In Vitro:
H2O : 2.5 mg/mL (11.45 mM; ultrasonic and warming and adjust pH to 10 with NaOH and heat to 60°C)
配制储备液
1 mM |
4.5819 mL |
22.9095 mL |
45.8190 mL |
5 mM |
0.9164 mL |
4.5819 mL |
9.1638 mL |
10 mM |
0.4582 mL |
2.2910 mL |
4.5819 mL |
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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参考文献 |
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